Knowledge: Lemon Lime (Citrus limon & Citrus microcarpa)

Citrus limon + Citrofortunella microcarpa

Lemon and lime are major fruit species from citrus family. The pulp and rind are commonly used in cooking and baking, while the juice (pH 2.2) gives refreshing sour taste to cuisine and beverages. The acidic juice of these citrus fruits is also used to improve food texture such as in macerating berries, tenderizing meat and to reduce greasiness of a dish.

The combination of lemon and lime gives a sour and slightly tangy sweet flavour that is suitable to be used as flavour enhancing ingredient in culinary. This combination offers greater source of nutritional values by providing rich source of Vitamin C. Extracts of these fruits were reported to have many beneficial effects including anticancer effects, antibacterial properties, reducing risk of hypertension, mental health problems, respiratory problems, arthritis, and rheumatism.

We provide practical, easy & consistent on food processing application.

Fresh natural flavor extract powder for Bakery & Beverage.

  • Vegan
  • Non-GMO
  • No Fillers
  • No Flavors
  • No Additives
  • No Artificial Colors
  • Soy and Gluten Free
  • Lab Tested for Authentic & Active Compound
  • Origin from Malaysia Borneo Forest
Bionutricia Lemon Lime Extract BRAND X
Certification Registered with the Ministry of Health, Halal Tested Not registered & certified
Raw Ingredients Lemon Citrus limon & Lime Citrus microcarpa from Malaysia farm Very little / no raw ingredients; Use non-Lemon Calamansi materials
Process Extracted through R.O. water & Ultra-sonic; Full bio-active ingredients Grinded peel powder; Extracted through ethanol process
Active Compound 0.5-1% Bioflavonoids Not Tested
Formulation Only Lemon Lime which is proven safe and effective Combination of other ingredients (effectiveness & safety is not proven); Added with prohibited drugs
Label Transparent, standardized Lemon Citrus limon & Lime Citrus microcarpa extract 0.5-1% bioflavonoids Misleading claim (quantity), incorrect amount of Lemon Calamansi extract, mostly filler ingredients

High Standard Product Quality Control​


Improves collagen synthesis

In vitro studies of Ascorbic acid
Subject Human skin fibroblast cell line
Duration of the study 5 days
  1. Control
  2. 100 µM AA
Parameters analysed Collagen I and IV expression level and synthesis of type I procollagen
Outcomes Vitamin C enhanced the expression levels of collagen I and IV in human skin fibroblast and increased the type I procollagen synthesis.

Table 1: Effect of ascorbic acid (AA) on type I and IV collagen expression level in human fibroblast cells.
Legends: white bar = without AA; black bar = with 100 µM AA
Table 2: Effect of ascorbic acid (AA) on type I procollagen synthesis in human fibroblast cells.
Legends: white bar = without AA; black bar = with 100 µM AA
Function -Vitamin C is essential for collagen biosynthesis.
-High content of Vitamin C and flavonoids act as good source of antioxidants, antibiotic and disinfectants which are beneficial for skin health maintenance.

Antitumor properties

In vivo studies of Citrus flavonoids
Subject Female Syrian golden hamsters (n = 60)
Duration of the study 1 week
  1. Control (DMSO)
  2. 2.5% hesperetin
  3. 2.5% neohesperidin
  4. 2.5% of naringenin
  5. 2.5% of naringin
Parameters analysed Average tumor burden in mm3
Outcomes All treatments with citrus flavonoids have reduced tumor burden in hamsters, and tumor volume reduction is significantly different between Group 1 and 5.

Group Average tumor burden (mm3)
1 269 ± 75
2 191 ± 47
3 222 ± 43
4 188 ± 55
5 77.1 ± 30

*Group 1 = untreated
*Group 2,3,4,5 = treated with citrus flavonoids

Function High content of flavonoids in citrus fruits may have antitumor properties and give protection effect against cancer.

Prevents kidney stone formation

Long-term lemonade therapy in patients
Subject Hypocitraturic nephrolithiasis patients (n = 32)
Duration of the study 24 hours (Urine analysis) >1 year (Stone formation)
  1. Control (Potassium Citrate)
  2. Lemonade
Parameters analysed Urinary citrate level and stone size
Outcomes -Lemonade therapy increased the urinary citrate level, similar to the effect of potassium citrate. -Stone formation rate was found decreased in patients with lemonade therapy. GroupControl (Potassium citrate)Lemonade

Table 1: Urinary profiles of control and lemonade therapy after 24 hours.
Urinary analysis
Pre-therapy Post-therapy Pre-therapy Post-therapy
Vol (mL) 2098 2488 1942 2354
Sodium (mg/day) 132 163 155 186
Uric acid (mg/day) 468 639 455 533
Oxalate (mg/day) 28 35 27 28
Citrate (mg/day) 349 831 350 733


Table 2: Stone size of control and lemonade therapy.
Mean of stone burden (mm2) 37.2 30.4
Function The high content of dietary citrate in citrus fruits can have citraturic effect and reduces the risk of kidney stone formation.

Reduces risk of inflammatory polyarthritis

Prospective, population-based, nested case-control study
Subject Individuals with and without inflammatory polyarthritis (IP) aged 45-74 years
Duration of the study 7 days
Groups (1) Control (n = 146) (2) Individuals with inflammatory polyarthritis (IP) (n = 73)
Parameters analysed Diet intake diary and development of inflammatory polyarthritis
Table 1: Association between dietary vitamin C and IP.
Association between dietary vitamin C and IP
Control group, no. (%) IP group, no. (%) Adjusted odd ratio (95% CI) p value
Vitamin C (< 40 mg) 10 (7) 15 (21) 3.9 folds 0.01 (significant difference)
Vitamin E *1, <5.3 mg *2, 5.3-7.7 mg *3, >7.7 mg *26 (36) *27 (37) *20 (27) *47 (32) *46 (32) *53 (36) *1.3 folds *1.6 folds *1fold 0.5 (no significant difference)

People who consumed less than 56 mg of vitamin C daily (above the current UK RNI of 40 mg/day) had a more than threefold increase in the risk of IP compared with those who consumed more.

Function Vitamin C is an important antioxidant micronutrient that may act as primary prevention of inflammatory polyarthritis.

Standardized Pure 20:1 Lemon Lime Powder Extract

  • Pure 20:1 Lemon Lime Extract
  • 0.5-1% Bioflavonoids (Bioactive Phytochemicals)
  • Ultrasonic solvent extraction
  • Rich in phytonutrient
  • High in antioxidant
  • For general health purpose
  • Promotes hydration
  • Good source of vitamin C
  • Supports weight loss
  • Promotes skin health
  • Aids digestion
  • Scientifically tested