Broccoli (Brassica oleracea var. italica)

Broccoli, Brassica oleracea, is an edible green plant whose large green color flowering head and stalk is eaten as a vegetable. China and India produced 73% of the world’s broccoli crops. It is eaten raw or cooked. By boiling, the characteristic sulfur-containing glucosinolate compound, sulforaphane can be easily diminished, but are better preserved by steaming, microwaving or stir-frying. With extraction at low temperature, the beneficial compounds can be concentrated and preserved in the form of powder that can be easily be taken as a food supplement. During processing or even chewing, the enzyme myrosinase can convert glucoraphanin present in broccoli into biologically active derivative sulforaphane.

Broccoli has a reputation as a superfood. It is low in calories but contains a wealth of nutrients and antioxidants (vitamin C, K, and A and dietary fiber) that support many aspects of human health. Broccoli has be found effective in certain cancerous conditions like prostate cancer, breast cancer and stomach cancer. The antioxidants present in broccoli can reduce inflammation, boost immune system and reduce the risk of cardiovascular disease.

Pure Broccoli Brassica oleracea Floret Extract must have Sulforaphane!

Without Sulforaphane, it’s not Broccoli Brassica oleracea Floret!
0.5-1% Sulforaphane (Bioactive Phytochemicals)

No harm/chemicals/preservative, just harmony, manufactured naturally

Vegan
Non-GMO
No Fillers
No Flavors
No Additives
No Preservatives
No Artificial Colors
Soy and Gluten Free
Lab Tested for Authentic & Active Compound
Origin from Malaysia Borneo Forest

Healthy Benefits

May improve brain
May control inflammation
High in antioxidant
Rich in phytonutrient
Ultrasonic hot water extraction
Scientifically tested

	BIONUTRICIA Brassica oleracea Floret Broccoli Extract	BRAND X
Certification	Registered with the Ministry of Health, Halal Tested	Not registered & certified
Raw Ingredients	Brassica oleracea Floret Broccoli Extract from Malaysia	Very little / no raw ingredients; Use non-Broccoli materials
Process	Extracted through R.O. water & Ultra-sonic; Full bio-active ingredients	Grinded plant powder; Extracted through ethanol process
Active Compound	Tested with high amount of Acetogenins	Not Tested
Formulation	Only Brassica oleracea Floret Broccoli- which is proven safe and effective	Combination of other ingredients (effectiveness & safety is not proven); Added with prohibited drugs
Label	Transparent, standardized Brassica oleracea Floret Broccoli Extract 0.5-1% Sulforaphane (Bioactive Phytochemicals)	Misleading claim (quantity), incorrect amount of Broccoli extract, mostly filler ingredients

SIRIM Test Report of Broccoli Extract

High Standard Product Quality Control

HEALTH BENEFITS

Lung Health

In vivo study on effect of broccoli on smokers
Subject	Healthy young smokers (n=27)
Duration of the study	10 days
Dosage	250g steamed broccoli per day
Groups	Before After
Parameters analysed	Survival rate, tumor growth, histopathologic and mechanism study
Function LG extract has both hypoglycemic and hypolipidemic effects.	Visible benefits 20 mg/kg of extract resulted in prolonged survival rates and increased inhibition of tumor growth and lymph node metastasis. The extract elevated apoptotic cell death and increased expression of active caspase-3 and -9 of cancer cells.

Anti-cancer

In vivo study on effect of broccoli on prostate cancer
Subject	Men having risk of prostate cancer (n=49)
Duration of the study	1 year
Dosage	400g broccoli per week
Groups	Broccoli-rich diet Pea-rich diet
Parameters analysed	Tumor size, histopathologic and mechanism study
Function α-mangostin and the G. mangostana xanthones are potential anti-colon cancer candidate.	Visible benefits The xanthone extract has cytotoxicity comparable to the drug and 2 times higher selectivity towards the colon cancer cells compared to normal cells. The extract and α-mangostin inhibited 3 key steps in tumor metastasis including the cell migration, cell invasion and clonogenicity.

Anti-diabetes

In vivo study on effect of broccoli on diabetes
Subject	Type 2 diabetic patients (n=81)
Duration of the study	4 weeks
Dosage	5, 10 g/day broccoli powder (BP)
Groups	10 g/d BP 5 g/d BP Placebo
Parameters analysed	Serum high-sensitive C reactive protein (hs-CRP), interleukin-6 (IL-6) and tumor necrosis α (TNF-α) levels. Cardiovascular risk factor parameters
Function High-sulforaphane broccoli had favorable effects on inflammatory markers and lipid profiles in diabetes.	Visible benefits BP treatment groups have a significant decrease in serum hs-CRP concentration (20.5% and 16.4%), decrease in serum IL-6 (3.6% and 0.7%), and TNF-α concentration (1.6% and 10.9%). BP in dose of 10 g/d, significantly decreased serum triglycerides, OX-LDL/LDL ratio and atherogenic index of plasma and increased HDL-C concentration.

Anti-inflammation

In vivo study on effect of broccoli on inflammation
Subject	Healthy overweight individuals (n=40)
Duration of the study	10 weeks
Dosage	30g/day broccoli
Groups	With treatment Without treatment
Parameters analysed	BMI Levels of TNF-α, IL-6, IL-1β and C-reactive protein
Function Long term consumption of broccoli is beneficial to reduce inflammation in obese individuals.	Visible benefits IL-6 levels significantly decreased (from 4.76 pg/mL to 2.11 pg/mL) during treatment and maintained at low grade (1.20-2.66 pg/mL) without treatment. C-reactive protein significantly decreased.

Prevent breast cancer

In vitro and in vivo study on effect of broccoli on breast cancer
Subject	Human breast cancer cells, xenograft tumor
Duration of the study	2 weeks
Dosage	50mg/kg sulforaphane
Groups	Control Treatment
Parameters analysed	Protein expression, cancer cell distribution
Function Sulforaphane is potentially used for the chemoprevention of breast cancer stem cells.	Visible benefits Sulforaphane decreased aldehyde dehydrogenase–positive cell population by 65% to 80% in human breast cancer cells and reduced the size and number of primary mammospheres by 8- to 125-fold and 45% to 75%, respectively.

Immunity

In vivo study on effect of broccoli on immunity
Subject	Young and old mice (n=18)
Duration of the study	11 days
Dosage	Sulforaphane (9 μmol/d per mouse)
Groups	Young Old Old + SFN
Parameters analysed	Immunity cell levels Glutathione (GSH) levels
Function Broccoli is capable to interfere with the age-related decline in immune function.	Visible benefits Systemic SFN administration reversed the decline in cellular immune function in old mice through Nrf2-mediated antioxidant enzyme expression and GSH synthesis to the level of younger mice.

Brain Health

In vivo study on effect of broccoli on brain health
Subject	Schzophrenia patients (n=10)
Duration of the study	8 weeks
Dosage	Sulforaphane-glucosinolate (30mg per day)
Groups	Before After
Parameters analysed	Cognitive function
Function SFN has the potential to improve cognitive function in patients with schizophrenia.	Visible benefits The mean score in the learning task increased significantly after the treatment.